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ProductsPlasmid kits Gel Extraction PCR Cleanup Our Technology Free Samples Orders Contact AdBiotec | . |
![]() . Plasmid purification kits are available in one size: . PPK-1 will process 100 bacterial samples. . Buy 2 or more kits and save. Yes, get two or more kits at the bulk purchase price of A$195 per kit (that's just $1.95/sample). . Each kit is supplied with spin columns fitted into 2ml receiver tubes, an equal number of 1.7 ml receiver/storage tubes, chemistry and instructions. . Experienced users: AdBiotec plasmid purification kits produce at least 30ug of sequencing grade DNA (typically 750 bases will be readable), yet at a significantly lower price than our major competitors. . . AdBiotec Plasmid DNA preparations are consistent in yield and quality . Ten plasmid preparations of pUC19 containing an insert with multiple HindIII sites were carried out simultaneously from 10 separate 1.5ml cultures of E.coli using the AdBiotec Plasmid DNA Purification Kit. .
.Panel A Undigested DNA. . Panel B The 10 plasmid DNA preps after digestion with 1unit HindIII for 1 hour at 37oC. Each track was loaded with 3uL DNA. . The procedure employs a standard alkaline method for the lysis of bacterial cells. After centrifugation, the cleared lysate is applied directly onto the spin column. Plasmid DNA is selectively bound to the triple layer silica membrane with a binding capacity of up to 30ug DNA. Highly pure plasmid DNA is eluted in TE buffer or water. The whole procedure is completed in less than 15 minutes. Plasmid DNA quality matches CsCl purified DNA. . . Applications include: automated fluorescent and radioactive sequencing, restriction analysis, ligation, PCR template preparation and DNA labelling. . . ![]() ![]() ![]() | . |